PDF DatasheetHuman EIF5A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant EIF5A based upon sequence from Human
Host: QP11775 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of EIF5A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11775.
Bioactivity Data: Untested
Amino Acid Sequence: MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The EIF5A protein solution (1 mg/ml) contains 50mM Tris-HCl, pH 7.5 and 10% Glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human EIF5A General Information | |
|---|---|
| Alternate Names | |
| EIF-5A, EIF5A1, eIF5AI, MGC99547, MGC104255, EIF5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-4D, Rev-binding factor. | |
| Molecular Weight | |
| 16.8 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P63241 |
| General Description of Recombinant Human EIF5A. | |
| EIF5A produced in E. Coli is a single, non-glycosylated polypeptide chain containing 154 amino acids and having a molecular mass of 16.8 kDa. EIF5A is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
Inhibition of Deoxyhypusine Synthase Enhances Islet b Cell Function and Survival in the Setting of Endoplasmic Reticulum Stress and Type 2 DiabetesPublication: Journal of Biological Chemistry 285.51 (2010): 39943-39952.Link: http://www.jbc.org/content/285/51/39943.full
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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