PDF DatasheetHuman EMC2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant EMC2 based upon sequence from Human
Host: QP11781 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of EMC2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11781.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAKVSEL YDVTWEEMRD KMRKWREENS RNSEQIVEVG EELINEYASK LGDDIWIIYE QVMIAALDYG RDDLALFCLQ ELRRQFPGSH RVKRLTGMRF EAMERYDDAI QLYDRILQED PTNTAARKRK IAIRKAQGKN VEAIRELNEY LEQFVGDQEA WHELAELYIN EHDYAKAAFC LEELMMTNPH NHLYCQQYAE VKYTQGGLEN LELSRKYFAQ ALKLNNRNMR ALFGLYMSAS HIASNPKASA KTKKDNMKYA SWAASQINRA YQFAGRSKKE TKYSLKAVED MLETLQITQS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The EMC2 solution contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 2mM DTT and 40% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human EMC2 General Information | |
|---|---|
| Alternate Names | |
| ER Membrane Protein Complex Subunit 2, KIAA0103, Tetratricopeptide Repeat Domain 35, Tetratricopeptide Repeat Protein 35, TPR Repeat Protein 35, TTC35. | |
| Molecular Weight | |
| 37.2 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q15006 |
| General Description of Recombinant Human EMC2. | |
| Human EMC2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-297) and having a molecular mass of 37.2kDa. EMC2 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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