PDF DatasheetHuman ENO2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ENO2 based upon sequence from Human
Host: QP11788 protein expressed in E. Coli, Native Protein.
Available Tags: His, Untagged
Protein Construction: A cDNA sequence encoding the sequence of ENO2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11788.
Bioactivity Data: Untested
Amino Acid Sequence: MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL
Purity: Greater than 95% as determined by SDS-PAGE. Single band on Western Blot.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Enolase 2 is supplied in 10mM Tris-HCl (pH 8), 250mM NaCl, 0.5mM DTT, 1.5mM Cysteine, and 50% Glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human ENO2 General Information | |
|---|---|
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| Alternate Names | |
| Gamma-enolase, EC 4.2. 1.11, 2-phospho-D-glycerate hydro-lyase, Neural enolase, Neuron-specific enolase, NSE, Enolase 2, ENO2. | |
| Molecular Weight | |
| 47.3 kDa | |
| Chromosomal Location | |
| p13.31 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ENO2 |
| Entrez Gene ID | 2026 |
| UniProt ID(s) | P09104 |
| COSMIC ID Link(s) | ENO2 |
| KEGG Gene ID(s) | hsa:2026 |
| General Description of Recombinant Human ENO2. | |
| Human Neuron Specific Enolase is expressed in E. coli containing 433 amino acids 2-434 fused to an amino terminal hexahistidine tag. The NSE is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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