PDF DatasheetPorcine Enterokinase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Enterokinase based upon sequence from Porcine
Host: QP11791 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Enterokinase was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25mM Tris-HCl, pH 7.6, 50mM NaCl, and 2mM CaCl2.
Bioactivity Data: Untested
Amino Acid Sequence: MGSKRIIPSR HRSLSTYEVM FTALFAILMV LCAGLIAVSW LTIKGSEKDA ALGKSHEARG TMKITSGVTY NPNLQDKLSV DFKVLAFDIQ QMIGEIFQSS NLKNEYKNSR VLQFENGSVI VIFDLLFAQW VSDENIKEEL IQGIEANKSS QLVAFHIDVN SIDITESLEN YSTTSPSTTS DKLTTSSPPA TPGNVSIECL PGSRPCADAL KCIAVDLFCD GELNCPDGSD EDSKICATAC DGKFLLTESS GSFDAAQYPK LSEASVVCQW IIRVNQGLSI ELNFSYFNTY SMDVLNIYEG VGSSKILRAS LWLMNPGTIR IFSNQVTVTF LIESDENDYI GFNATYTAFN STELNNDEKI NCNFEDGFCF WIQDLNDDNE WERIQGTTFP PFTGPNFDHT FGNASGFYIS TPTGPGGRQE RVGLLSLPLE PTLEPVCLSF WYYMYGENVY KLSINISNDQ NIEKIIFQKE GNYGENWNYG QVTLNETVEF KVAFNAFKNQ FLSDIALDDI SLTYGICNVS LYPEPTLVPT SPPELPTDCG GPFELWEPNT TFTSMNFPNN YPNQAFCVWN LNAQKGKNIQ LHFEEFDLEN IADVVEIRDG EEDDSLLLAV YTGPGPVEDV FSTTNRMTVL FITNDALTKG GFKANFTTGY HLGIPEPCKE DNFQCENGEC VLLVNLCDGF SHCKDGSDEA HCVRFLNGTA NNSGLVQFRI QSIWHTACAE NWTTQTSDDV CQLLGLGTGN SSMPFFSSGG GPFVKLNTAP NGSLILTASE QCFEDSLILL QCNHKSCGKK QVAQEVSPKI VGGNDSREGA WPWVVALYYN GQLLCGASLV SRDWLVSAAH CVYGRNLEPS KWKAILGLHM TSNLTSPQIV TRLIDEIVIN PHYNRRRKDS DIAMMHLEFK VNYTDYIQPI CLPEENQVFP PGRICSIAGW GKVIYQGSPA DILQEADVPL LSNEKCQQQM PEYNITENMM CAGYEEGGID SCQGDSGGPL MCLENNRWLL AGVTSFGYQC ALPNRPGVYA RVPKFTEWIQ SFLH
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: 2 IU/µl, 50mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.
Storage Conditions: One year when stored at -20°C, one week at room temperature.
Physical Appearance: Sterile Liquid.
| Recombinant Porcine Enterokinase General Information | |
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| Alternate Names | |
| Enteropeptidase, EC 3.4. 21.9, Enterokinase, Serine protease 7, ENTK, MGC133046. | |
| Molecular Weight | |
| 114.8 kDa | |
| Chromosomal Location | |
| On chromosome 13 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TMPRSS15 |
| Entrez Gene ID | 397152 |
| UniProt ID(s) | P98074 |
| COSMIC ID Link(s) | TMPRSS15 |
| KEGG Gene ID(s) | ssc:397152 |
| General Description of Recombinant Porcine Enterokinase. | |
| Porcine enteropeptidase is a specific protease which cleaves after the lysine at its recognition site: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave a site followed by proline. Theoretical MW is 21,880 Dalton, the apparent MW on SDS-PAGE is about 40 kDa. If a fusion tag is located in the N-terminus with an enterokinase site, enterokinase will be able to remove the fusion tag and to generate the protein exactly as you need without adding any unwanted residues. enQuire Bios enterokinase is a highly purified enterokinase from porcine. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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