PDF DatasheetBovine Enterokinase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Enterokinase based upon sequence from Bovine
Host: QP11793 protein expressed in Pichia Pastoris, E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Enterokinase was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): One unit is defined as the amount of enzyme needed to cleave 50µg of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25mM Tris-HCl, pH 7.6, 50mM NaCl, and 2mM CaCl2.
Other Applications: Each reaction contains 50 µg of partially purified thioredoxin-NP-27 fusion protein and varying amounts of Bovine EK. Reactions were incubated at 25°C for 16 hours and analyzed on a Coomassiestained 15% SDS gel.
Bioactivity Data: Untested
Amino Acid Sequence: MGSKRSVPSR HRSLTTYEVM FAVLFVILVA LCAGLIAVSW LSIQGSVKDA AFGKSHEARG TLKIISGATY NPHLQDKLSV DFKVLAFDIQ QMIDDIFQSS NLKNEYKNSR VLQFENGSII VIFDLLFDQW VSDKNVKEEL IQGIEANKSS QLVTFHIDLN SIDITASLEN FSTISPATTS EKLTTSIPLA TPGNVSIECP PDSRLCADAL KCIAIDLFCD GELNCPDGSD EDNKTCATAC DGRFLLTGSS GSFEALHYPK PSNNTSAVCR WIIRVNQGLS IQLNFDYFNT YYADVLNIYE GMGSSKILRA SLWSNNPGII RIFSNQVTAT FLIQSDESDY IGFKVTYTAF NSKELNNYEK INCNFEDGFC FWIQDLNDDN EWERTQGSTF PPSTGPTFDH TFGNESGFYI STPTGPGGRR ERVGLLTLPL DPTPEQACLS FWYYMYGENV YKLSINISSD QNMEKTIFQK EGNYGQNWNY GQVTLNETVE FKVSFYGFKN QILSDIALDD ISLTYGICNV SVYPEPTLVP TPPPELPTDC GGPHDLWEPN TTFTSINFPN SYPNQAFCIW NLNAQKGKNI QLHFQEFDLE NIADVVEIRD GEGDDSLFLA VYTGPGPVND VFSTTNRMTV LFITDNMLAK QGFKANFTTG YGLGIPEPCK EDNFQCKDGE CIPLVNLCDG FPHCKDGSDE AHCVRLFNGT TDSSGLVQFR IQSIWHVACA ENWTTQISDD VCQLLGLGTG NSSVPTFSTG GGPYVNLNTA PNGSLILTPS QQCLEDSLIL LQCNYKSCGK KLVTQEVSPK IVGGSDSREG AWPWVVALYF DDQQVCGASL VSRDWLVSAA HCVYGRNMEP SKWKAVLGLH MASNLTSPQI ETRLIDQIVI NPHYNKRRKN NDIAMMHLEM KVNYTDYIQP ICLPEENQVF PPGRICSIAG WGALIYQGST ADVLQEADVP LLSNEKCQQQ MPEYNITENM VCAGYEAGGV DSCQGDSGGP LMCQENNRWL LAGVTSFGYQ CALPNRPGVY ARVPRFTEWI QSFLH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Bovine EK in 50mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.
Storage Conditions: One year when stored at 20°C. Please avoid freeze-thaw cycles.
Physical Appearance: Sterile liquid solution.
| Recombinant Bovine Enterokinase General Information | |
|---|---|
| Alternate Names | |
| Enteropeptidase, EC 3.4. 21.9, Enterokinase, Serine protease 7, ENTK, MGC133046. | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P98072 |
| General Description of Recombinant Bovine Enterokinase. | |
| Enterokinase (rEK) Bovine Recombinant is the catalytic subunit of bovine enterokinase, which is expressed by the yeast and purified to yield a high enzyme activity preparation. EK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence. Recombinant Bovine Enterokinase is a single glycosylated polypeptide chain containing 235 amino acids and having an MW of ~43kDa. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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