PDF DatasheetHuman FBL Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant FBL based upon sequence from Human
Host: QP11853 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of FBL was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11853.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH RSMGKNVMVE PHRHEGVFIC RGKEDALVTK NLVPGESVYG EKRVSISEGD DKIEYRAWNP FRSKLAAAIL GGVDQIHIKP GAKVLYLGAA SGTTVSHVSD IVGPDGLVYA VEFSHRSGRD LINLAKKRTN IIPVIEDARH PHKYRMLIAM VDVIFADVAQ PDQTRIVALN AHTFLRNGGH FVISIKANCI DSTASAEAVF ASEVKKMQQE NMKPQEQLTL EPYERDHAVV VGVYRPPPKV KN
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The FBL solution (0.5 mg/ml) contains Phosphate buffered saline (pH 7.4), 30% glycerol, and 1mM EDTA.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human FBL General Information | |
|---|---|
| Alternate Names | |
| rRNA 2′-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, FBL, FIB1, FLRN, fibrillarin, FIB, RNU3IP1. | |
| Molecular Weight | |
| 28.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P22087 |
| General Description of Recombinant Human FBL. | |
| Human FBL fused with 23 amino acid His tag at N-terminus produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 262 amino acids (83-321 AA ) and having a molecular mass of 28.9kDa. The FBL is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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