PDF DatasheetHuman FUCA1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant FUCA1 based upon sequence from Human
Host: QP11909 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of FUCA1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11909.
Bioactivity Data: Untested
Amino Acid Sequence: VRRAQPPRRY TPDWPSLDSR PLPAWFDEAK FGVFIHWGVF SVPAWGSEWF WWHWQGEGRP QYQRFMRDNY PPGFSYADFG PQFTARFFHP EEWADLFQAA GAKYVVLTTK HHEGFTNWPS PVSWNWNSKD VGPHRDLVGE LGTALRKRNI RYGLYHSLLE WFHPLYLLDK KNGFKTQHFV SAKTMPELYD LVNSYKPDLI WSDGEWECPD TYWNSTNFLS WLYNDSPVKD EVVVNDRWGQ NCSCHHGGYY NCEDKFKPQS LPDHKWEMCT SIDKFSWGYR RDMALSDVTE ESEIISELVQ TVSLGGNYLL NIGPTKDGLI VPIFQERLLA VGKWLSINGE AIYASKPWRV QWEKNTTSVW YTSKGSAVYA IFLHWPENGV LNLESPITTS TTKITMLGIQ GDLKWSTDPD KGLFISLPQL PPSAVPAEFA WTIKLTGVKH HHHHH
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: FUCA1 protein solution (0.5 mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human FUCA1 General Information | |
|---|---|
| Alternate Names | |
| Fucosidase, Alpha-L- 1, Tissue, Alpha-L-Fucoside Fucohydrolase 1, Alpha-L-Fucosidase 1, Alpha-L-Fucosidase I, EC 3.2. 1.51, Tissue Alpha-L-Fucosidase, EC 3.2. 1, FUCA, Tissue alpha-L-fucosidase. | |
| Molecular Weight | |
| 51.7 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P04066 |
| General Description of Recombinant Human FUCA1. | |
| FUCA1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 445 amino acids (28-466AA ) and having a molecular mass of 51.7kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). FUCA1 is expressed with a 6 amino acid His tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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