PDF DatasheetHuman GAD1 iso1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GAD1 iso1 based upon sequence from Human
Host: QP11918 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GAD1 iso1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11918.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASSTPS SSATSSNAGA DPNTTNLRPT TYDTWCGVAH GCTRKLGLKI CGFLQRTNSL EEKSRLVSAF KERQSSKNLL SCENSDRDAR FRRTETDFSN LFARDLLPAK NGEEQTVQFL LEVVDILLNY VRKTFDRSTK VLDFHHPHQL LEGMEGFNLE LSDHPESLEQ ILVDCRDTLK YGVRTGHPRF FNQLSTGLDI IGLAGEWLTS TANTNMFTYE IAPVFVLMEQ ITLKKMREIV GWSSKDGDGI FSPGGAISNM YSIMAARYKY FPEVKTKGMA AVPKLVLFTS EQSHYSIKKA GAALGFGTDN VILIKCNERG KIIPADFEAK ILEAKQKGYV PFYVNATAGT TVYGAFDPIQ EIADICEKYN LWLHVDAAWG GGLLMSRKHR HKLNGIERAN SVTWNPHKMM GVLLQCSAIL VKEKGILQGC NQMCAGYLFQ PDKQYDVSYD TGDKAIQCGR HVDIFKFWLM WKAKGTVGFE NQINKCLELA EYLYAKIKNR EEFEMVFNGE PEHTNVCFWY IPQSLRGVPD SPQRREKLHK VAPKIKALMM ESGTTMVGYQ PQGDKANFFR MVISNPAATQ SDIDFLIEEI ERLGQDL
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: GAD1 iso1 protein solution (0.5 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human GAD1 iso1 General Information | |
|---|---|
 |
|
| Alternate Names | |
| Glutamate Decarboxylase 1 (Brain, 67kDa), Glutamate Decarboxylase 67 KDa Isoform, 67 KDa Glutamic Acid Decarboxylase, EC 4.1. 1.15, GAD-67, CPSQ1, SCP, GAD, Glutamate Decarboxylase 1 (Brain, 67kD), Glutamate Decarboxylase 1, EC 4.1. 1, GAD67, Glutamate decarboxylase 1. | |
| Molecular Weight | |
| 69.3 kDa | |
| Chromosomal Location | |
| q31.1 on chromosome 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GAD1 |
| Entrez Gene ID | 2571 |
| UniProt ID(s) | Q99259 |
| COSMIC ID Link(s) | GAD1 |
| KEGG Gene ID(s) | hsa:2571 |
| General Description of Recombinant Human GAD1 iso1. | |
| Human GAD1 iso1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 617 amino acids (1-594 AA) and having a molecular mass of 69.3kDa. GAD1 iso1 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.