PDF DatasheetHuman GAGE12F Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GAGE12F based upon sequence from Human
Host: QP11923 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GAGE12F was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11923.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSWRGRS TYYWPRPRRY VQPPEMIGPM RPEQFSDEVE PATPEEGEPA TQRQDPAAAQ EGEDEGASAG QGPKPEAHSQ EQGHPQTGCE CEDGPDGQEM DPPNPEEVKT PEEGEKQSQC
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: GAGE12F protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human GAGE12F General Information | |
|---|---|
| Alternate Names | |
| G Antigen 12F, GAGE-12F, G Antigen 12F/G/I. | |
| Molecular Weight | |
| 15.4 kDa | |
| Chromosomal Location | |
| p11.23 on chromosome X | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GAGE12F |
| Entrez Gene ID | 100008586; 645073; 26748; 2579 |
| UniProt ID(s) | P0CL80 |
| COSMIC ID Link(s) | GAGE12F |
| KEGG Gene ID(s) | hsa:645073; hsa:26748; hsa:2579; hsa:100008586 |
| General Description of Recombinant Human GAGE12F. | |
| Human GAGE12F produced in E. Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids (1-117aa) and having a molecular mass of 15.4kDa. GAGE12F is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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