PDF DatasheetMouse GDF15 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GDF15 based upon sequence from Mouse
Host: QP11948 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GDF15 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11948.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSAHAHPR DSCPLGPGRC CHLETVQATL EDLGWSDWVL SPRQLQLSMC VGECPHLYRS ANTHAQIKAR LHGLQPDKVP APCCVPSSYT PVVLMHRTDS GVSLQTYDDL VARGCHCA
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: GDF15 protein solution (1.0 mg/ml) containing 20mM Phosphate buffer (pH 8.0) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Mouse GDF15 General Information | |
|---|---|
| Alternate Names | |
| Growth/differentiation factor 15, GDF-15. | |
| Molecular Weight | |
| 14.9 kDa | |
| Chromosomal Location | |
| B3.3 on chromosome 8 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Gdf15 |
| Entrez Gene ID | 23886 |
| UniProt ID(s) | Q9Z0J7 |
| COSMIC ID Link(s) | Gdf15 |
| KEGG Gene ID(s) | mmu:23886 |
| General Description of Recombinant Mouse GDF15. | |
| Mouse GDF15 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 138 amino acids (189-303 AA) and having a molecular mass of 14.9kDa. GDF15 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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