PDF DatasheetHuman Leptin Protein Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Leptin Protein based upon sequence from Human
Host: QP1201 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human Leptin Protein, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP1201.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC
Purity: Greater than 97% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions: A Certificate of Analysis (CofA) will be sent along with the protein and will contain the proper reconstitution instructions.
Concentration of Human Leptin Protein Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Leptin Protein Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LEP |
| Entrez Gene ID | 3952 |
| Ensemble Gene ID | ENSG00000174697 |
| RefSeq Protein Accession(s) | NP_000221.1 |
| RefSeq mRNA Accession(s) | NM_000230.2, XM_005250340.4 |
| UniProt ID(s) | P41159 |
| UniGene ID(s) | Hs.194236 |
| HGNC ID(s) | HGNC:6553 |
| COSMIC ID Link(s) | LEP |
| KEGG Gene ID(s) | hsa:3952 |
| PharmGKB ID(s) | PA228 |
| General Description of Recombinant Human Leptin Protein Protein. | |
| May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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