PDF DatasheetE. Coli GRXB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GRXB based upon sequence from E. Coli
Host: QP12054 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GRXB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12054.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: GRXB protein solution (1 mg/ml) 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 50mM NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant E. Coli GRXB General Information | |
|---|---|
| Alternate Names | |
| Glutaredoxin-2, Grx2, grxB, b1064, JW1051. | |
| Molecular Weight | |
| 26.5 kDa | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | grxB |
| Entrez Gene ID | 946926 |
| UniProt ID(s) | P0AC59 |
| COSMIC ID Link(s) | grxB |
| KEGG Gene ID(s) | eco:b1064; ecj:JW1051 |
| General Description of Recombinant E. Coli GRXB. | |
| GRXB produced in E. Coli is a single, non-glycosylated polypeptide chain containing 235 amino acids (1-215 AA ) and having a molecular mass of 26.5kDa. GRXB is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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