PDF DatasheetHuman GZMK Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GZMK based upon sequence from Human
Host: QP12073 protein expressed in Insect, E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GZMK was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12073.
Bioactivity Data: Untested
Amino Acid Sequence: ADPIIGGKEV SPHSRPFMAS IQYGGHHVCG GVLIDPQWVL TAAHCQYRFT KGQSPTVVLG AHSLSKNEAS KQTLEIKKFI PFSRVTSDPQ SNDIMLVKLQ TAAKLNKHVK MLHIRSKTSL RSGTKCKVTG WGATDPDSLR PSDTLREVTV TVLSRKLCNS QSYYNGDPFI TKDMVCAGDA KGQKDSCKGD SGGPLICKGV FHAIVSGGHE CGVATKPGIY TLLTKKYQTW IKSNLVPPHT NHHHHHH
Purity: Greater than 95.0% as determined by analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: GZMK protein solution (0.25 mg/ml) containing Phosphate Buffered Saline (pH 7.4), 20% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human GZMK General Information | |
|---|---|
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| Alternate Names | |
| GZMK, TRYP2, Granzyme-K, Granzyme K, Fragmentin-3, Granzyme-3, NK-tryptase-2, NK-Tryp-2. | |
| Molecular Weight | |
| 26.9 kDa | |
| Chromosomal Location | |
| q11.2 on chromosome 5 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GZMK |
| Entrez Gene ID | 3003 |
| UniProt ID(s) | P49863 |
| COSMIC ID Link(s) | GZMK |
| KEGG Gene ID(s) | hsa:3003 |
| General Description of Recombinant Human GZMK. | |
| Human GZMK produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 247 amino acids (27-264) and having a molecular mass of 26.9kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). GZMK is fused to a 6 amino acid IgG His-Tag at C-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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