PDF DatasheetHuman HAAO Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HAAO based upon sequence from Human
Host: QP12098 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HAAO was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12098.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMERRLG VRAWVKENRG SFQPPVCNKL MHQEQLKVMF IGGPNTRKDY HIEEGEEVFY QLEGDMVLRV LEQGKHRDVV IRQGEIFLLP ARVPHSPQRF ANTVGLVVER RRLETELDGL RYYVGDTMDV LFEKWFYCKD LGTQLAPIIQ EFFSSEQYRT GKPIPDQLLK EPPFPLSTRS IMEPMSLDAW LDSHHRELQA GTPLSLFGDT YETQVIAYGQ GSSEGLRQNV DVWLWQLEGS SVVTMGGRRL SLAPDDSLLV LAGTSYAWER TQGSVALSVT QDPACKKPLG
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: HAAO protein solution (0.5 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human HAAO General Information | |
|---|---|
| Alternate Names | |
| 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-HAO, 3-hydroxyanthranilic acid dioxygenase, HAD, HAAO, HAO. | |
| Molecular Weight | |
| 35 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P46952 |
| General Description of Recombinant Human HAAO. | |
| Human HAAO produced in E. Coli is a single, non-glycosylated polypeptide chain containing 310 amino acids (1-286 AA ) and having a molecular mass of 35kDa. HAAO is fused to a 24 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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