PDF DatasheetHuman HADH Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HADH based upon sequence from Human
Host: QP12099 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HADH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12099.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSSSTASAS AKKIIVKHVT VIGGGLMGAG IAQVAAATGH TVVLVDQTED ILAKSKKGIE ESLRKVAKKK FAENPKAGDE FVEKTLSTIA TSTDAASVVH STDLVVEAIV ENLKVKNELF KRLDKFAAEH TIFASNTSSL QITSIANATT RQDRFAGLHF FNPVPVMKLV EVIKTPMTSQ KTFESLVDFS KALGKHPVSC KDTPGFIVNR LLVPYLMEAI RLYERGDASK EDIDTAMKLG AGYPMGPFEL LDYVGLDTTK FIVDGWHEMD AENPLHQPSP SLNKLVAENK FGKKTGEGFY KYK
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The HADH solution contains 20mM Tris-HCl pH 8.0, 0.1M NaCl and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear colorless solution.
| Recombinant Human HADH General Information | |
|---|---|
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| Alternate Names | |
| EC 1.1. 1.35, HAD, HADH1, HHF4, MSCHAD, SCHAD, Hydroxyacyl-coenzyme A dehydrogenase, HCDH, Short-chain 3-hydroxyacyl-CoA dehydrogenase, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, HADH, HADHSC, MGC8392. | |
| Molecular Weight | |
| 35.1 kDa | |
| Chromosomal Location | |
| q25 on chromosome 4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HADH |
| Entrez Gene ID | 3033 |
| UniProt ID(s) | Q16836 |
| COSMIC ID Link(s) | HADH |
| KEGG Gene ID(s) | hsa:3033 |
| General Description of Recombinant Human HADH. | |
| Human HADH fused to a 21 amino acids His Tag at N-terminal produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 323 amino acids (13-314 AA ) and having a molecular mass of 35.1 kDa. The HADH is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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