PDF DatasheetHuman HDAC2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HDAC2 based upon sequence from Human
Host: QP12202 protein expressed in Insect, E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HDAC2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12202.
Bioactivity Data: Untested
Amino Acid Sequence: MAYSQGGGKK KVCYYYDGDI GNYYYGQGHP MKPHRIRMTH NLLLNYGLYR KMEIYRPHKA TAEEMTKYHS DEYIKFLRSI RPDNMSEYSK QMQRFNVGED CPVFDGLFEF CQLSTGGSVA GAVKLNRQQT DMAVNWAGGL HHAKKSEASG FCYVNDIVLA ILELLKYHQR VLYIDIDIHHGDGVEEAFYT TDRVMTVSFH KYGEYFPGTG DLRDIGAGKG KYYAVNFPMR DGIDDESYGQ IFKPIISKVM EMYQPSAVVL QCGADSLSGD RLGCFNLTVK GHAKCVEVVK TFNLPLLMLG GGGYTIRNVA RCWTYETAVA LDCEIPNELP YNDYFEYFGP DFKLHISPSN MTNQNTPEYMEKIKQRLFEN LRMLPHAPGV QMQAIPEDAV HEDSGDEDGE DPDKRISIRA SDKRIACDEE FSDSEDEGEG GRRNVADHKK GAKKARIEED KKETEDKKTD VKEEDKSKDN SGEKTDTKGT KSEQLSNPSR HHHHHH
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The HDAC2 solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl, 0.1mM PMSF and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human HDAC2 General Information | |
|---|---|
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| Alternate Names | |
| Histone deacetylase 2, YAF1, HD2, YY1-associated factor 1, transcriptional regulator homolog RPD3, RPD3, EC 3.5. 1.98. | |
| Molecular Weight | |
| 56.4 kDa | |
| Chromosomal Location | |
| q21 on chromosome 6 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HDAC2 |
| Entrez Gene ID | 3066 |
| UniProt ID(s) | Q92769 |
| COSMIC ID Link(s) | HDAC2 |
| KEGG Gene ID(s) | hsa:3066 |
| General Description of Recombinant Human HDAC2. | |
| Human HDAC2 produced in Hi-5 Cell is a single, non-glycosylated polypeptide chain containing 496 amino acids (1-488) and having a molecular mass of 56.4 kDa. The HDAC2 is fused to an 8 amino acid His-Tag at C-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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