PDF DatasheetHuman HERC5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HERC5 based upon sequence from Human
Host: QP12217 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HERC5 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12217.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSFDLTVRR NHLIEDVLNQ LSQFENEDLR KELWVSFSGE IGYDLGGVKK EFFYCLFAEM IQPEYGMFMY PEGASCMWFP VKPKFEKKRY FFFGVLCGLS LFNCNVANLP FPLALFKKLL DQMPSLEDLK ELSPDLGKNL QTLLDDEGDN FEEVFYIHFN VHWDRNDTNL IPNGSSITVN QTNKRDYVSK YINYIFNDSV KAVYEEFRRG FYKMCDEDII KLFHPEELKD VIVGNTDYDW KTFEKNARYE PGYNSSHPTI VMFWKAFHKL TLEEKKKFLV FLTGTDRLQM KDLNNMKITF CCPESWNERD PIRALTCFSV LFLPKYSTME TVEEALQEAI NNNRGFG
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: HERC5 protein solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human HERC5 General Information | |
|---|---|
| Alternate Names | |
| HERC5, HECT and RLD Domain Containing E3 Ubiquitin Protein Ligase 5, CEB1, Hect Domain and RLD 5, Cyclin-E-Binding Protein 1, CEBP1, HECT Domain and RCC1-Like Domain-Containing Protein 5, E3 ISG15–Protein Ligase HERC5, Probable E3 Ubiquitin-Protein Ligase HERC5, EC 6.3. 2. – , EC 6.3. 2. | |
| Molecular Weight | |
| 43 kDa | |
| Chromosomal Location | |
| q22.1 on chromosome 4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HERC5 |
| Entrez Gene ID | 51191 |
| UniProt ID(s) | Q9UII4 |
| COSMIC ID Link(s) | HERC5 |
| KEGG Gene ID(s) | hsa:51191 |
| General Description of Recombinant Human HERC5. | |
| Human HERC5 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 367 amino acids (681-1024 AA ) and having a molecular mass of 43kDa. HERC5 is fused to a 23 amino acid His-tag at N-terminus. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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