PDF DatasheetHuman HLA-C Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HLA-C based upon sequence from Human
Host: QP12273 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HLA-C was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12273.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSCSHSMRY FDTAVSRPGR GEPRFISVGY VDDTQFVRFD SDAASPRGEP RAPWVEQEGP EYWDRETQKY KRQAQADRVS LRNLRGYYNQ SEDGSHTLQR MSGCDLGPDG RLLRGYDQSA YDGKDYIALN EDLRSWTAAD TAAQITQRKL EAARAAEQLR AYLEGTCVEW LRRYLENGKE TLQRAEPPKT HVTHHPLSDH EATLRCWALG FYPAEITLTW QRDGEDQTQD TELVETRPAG DGTFQKWAAV VVPSGQEQRY TCHMQHEGLQ EPLTLSWEPS SQPTIPI
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The HLA-C solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human HLA-C General Information | |
|---|---|
| Alternate Names | |
| Major Histocompatibility Complex Class I- C, D6S204, HLA-JY3, PSORS1, HLC-C, HLA Class I Histocompatibility Antigen C Alpha Chain, Human Leukocyte Antigen-C Alpha Chain, Major Histocompatibility Antigen HLA-C, MHC Class I Antigen Heavy Chain HLA-C. | |
| Molecular Weight | |
| 34.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | NP_002108 |
| General Description of Recombinant Human HLA-C. | |
| Human HLA-C produced in E. coli is a single, non-glycosylated polypeptide chain containing 307 amino acids (25-308) and having a molecular mass of 34.9kDa. HLA-C is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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