PDF DatasheetHuman HLA-DRB1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HLA-DRB1 based upon sequence from Human
Host: QP12276 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HLA-DRB1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12276.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGDTRPRFLW QPKRECHFFN GTERVRFLDR YFYNQEESVR FDSDVGEFRA VTELGRPDAE YWNSQKDILE QARAAVDTYC RHNYGVVESF TVQRRVQPKV TVYPSKTQPL QHHNLLVCSV SGFYPGSIEV RWFLNGQEEK AGMVSTGLIQ NGDWTFQTLV MLETVPRSGE VYTCQVEHPS VTSPLTVEWR ARSESAQSK
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: HLA-DRB1 protein solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human HLA-DRB1 General Information | |
|---|---|
| Alternate Names | |
| DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1, MHC class II antigen DRB1 16, DR-16, DR16, Human Leucocyte AntigenDRB1, MHC Class IIHLA-DR-Beta Cell Surface Glycoprotein, MHC Class IIHLA-DRw10-Beta. | |
| Molecular Weight | |
| 25.2 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q29974 |
| General Description of Recombinant Human HLA-DRB1. | |
| Human HLA-DRB1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (30-227 AA) and having a molecular mass of 25.2kDa. HLA-DRB1 is fused to a 21 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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