PDF DatasheetHuman HLF Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HLF based upon sequence from Human
Host: QP12278 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HLF was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12278.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEKMSRPLPL NPTFIPPPYG VLRSLLENPL KLPLHHEDAF SKDKDKEKKL DDESNSPTVP QSAFLGPTLW DKTLPYDGDT FQLEYMDLEE FLSENGIPPS PSQHDHSPHP PGLQPASSAA PSVMDLSSRA SAPLHPGIPS PNCMQSPIRP GQLLPANRNT PSPIDPDTIQ VPVGYEPDPA DLALSSIPGQ EMFDPRKRKF SEEELKPQPM IKKARKVFIP DDLKDDKYWA RRRKNNMAAK RSRDARRLKE NQIAIRASFL EKENSALRQE VADLRKELGK CKNILAKYEA RHGPL
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: HLF protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 40% glycerol, 0.2M NaCl and 0.1mM PMSF.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human HLF General Information | |
|---|---|
| Alternate Names | |
| Hepatic leukemia factor, HLF. | |
| Molecular Weight | |
| 35.3 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q16534 |
| General Description of Recombinant Human HLF. | |
| Human HLF produced in E. Coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (1-295 AA ) and having a molecular mass of 35.3kDa. HLF is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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