PDF DatasheetHuman HMGA1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HMGA1 based upon sequence from Human
Host: QP12280 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HMGA1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12280.
Bioactivity Data: Untested
Amino Acid Sequence: MSESSSKSSQ PLASKQEKDG TEKRGRGRPR KQPPVSPGTA LVGSQKEPSE VPTPKRPRGR PKGSKNKGAA KTRKTTTTPG RKPRGRPKKL EKEEEEGISQ ESSEEEQLEH HHHHH
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: HMGA1 protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50% glycerol and 0.2M NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human HMGA1 General Information | |
|---|---|
| Alternate Names | |
| High mobility group protein HMG-I/HMG-Y, HMG-I(Y), High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein R , HMGA1, HMGIY, HMG-R, HMGA1A. | |
| Molecular Weight | |
| 12.7 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P17096 |
| General Description of Recombinant Human HMGA1. | |
| Human HMGA1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 115 amino acids (1-107 AA ) and having a molecular mass of 12.7kDa (Molecular weight on SDS-PAGE will appear higher). HMGA1 is fused to an 8 amino acid His-tag at C-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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