PDF DatasheetHuman HMGB2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HMGB2 based upon sequence from Human
Host: QP12282 protein expressed in Baculovirus.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HMGB2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12282.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH TGSMGKGDPN KPRGKMSSYA FFVQTCREEH KKKHPDSSVN FAEFSKKCSE RWKTMSAKEK SKFEDMAKSD KARYDREMKN YVPPKGDKKG KKKDPNAPKR PPSAFFLFCS EHRPKIKSEH PGLSIGDTAK KLGEMWSEQS AKDKQPYEQK AAKLKEKYEK DIAAYRAKGK SEAGKKGPGR PTGSKKKNEP EDEEEEEEEE DEDEEEEDED EE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The HMGB2 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 30% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human HMGB2 General Information | |
|---|---|
| Alternate Names | |
| High mobility group (nonhistone chromosomal) protein B2, h mobility group box 2, HMG2. | |
| Molecular Weight | |
| 26.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P26583 |
| General Description of Recombinant Human HMGB2. | |
| Human HMGB2 produced in Baculovirus is a single polypeptide chain containing 232 amino acids (1-209) and having a molecular mass of 26.4 kDa. The HMGB2 is fused to a 23 amino acid His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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