PDF DatasheetHuman HPCAL1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HPCAL1 based upon sequence from Human
Host: QP12298 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HPCAL1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12298.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVLQDLRENT EFTDHELQEW YKGFLKDCPT GHLTVDEFKK IYANFFPYGD ASKFAEHVFR TFDTNGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISRSE MLEIVQAIYK MVSSVMKMPE DESTPEKRTD KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQF
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The HPCAL1 protein solution (1 mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 0.2M NaCl and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human HPCAL1 General Information | |
|---|---|
| Alternate Names | |
| Hippocalcin-Like 1, HLP2, BDR1, Calcium-Binding protein BDR-1, Visinin-Like protein 3, VILIP-3 | |
| Molecular Weight | |
| 24.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P37235 |
| General Description of Recombinant Human HPCAL1. | |
| Human HPCAL1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 213amino acids (1-193AA ) and having a molecular weight of 24.4kDa. The HPCAL1 is fused to 20AA His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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