PDF DatasheetHuman HPGD Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HPGD based upon sequence from Human
Host: QP12301 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HPGD was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12301.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MHVNGKVALV TGAAQGIGRA FAEALLLKGA KVALVDWNLE AGVQCKAALD EQFEPQKTLF IQCDVADQQQ LRDTFRKVVD HFGRLDILVN NAGVNNEKNW EKTLQINLVS VISGTYLGLD YMSKQNGGEG GIIINMSSLA GLMPVAQQPV YCASKHGIVG FTRSAALAAN LMNSGVRLNA ICPGFVNTAI LESIEKEENM GQYIEYKDHI KDMIKYYGIL DPPLIANGLI TLIEDDALNG AIMKITTSKG IHFQDYDTTP FQAKTQ
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The HPGD protein solution (0.5 mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 0.1M NaCl,1mM DTT and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human HPGD General Information | |
|---|---|
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| Alternate Names | |
| GDH1, SDR36C1, 15-PGDH, PGDH, Prostaglandin dehydrogenase 1, hydroxyprostaglandin dehydrogenase 15-(NAD) | |
| Molecular Weight | |
| 31.1 kDa | |
| Chromosomal Location | |
| q34.1 on chromosome 4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HPGD |
| Entrez Gene ID | 3248 |
| UniProt ID(s) | P15428 |
| COSMIC ID Link(s) | HPGD |
| KEGG Gene ID(s) | hsa:3248 |
| General Description of Recombinant Human HPGD. | |
| HPGD Recombinant E. coli produced in E. Coli is a single, non-glycosylated polypeptide chain containing 286 amino acids (1-266AA ) and having a molecular mass of 31.1 kDa. HPGD is fused to a 20 amino acids His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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