PDF DatasheetHuman HPSE Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HPSE based upon sequence from Human
Host: QP12304 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HPSE was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12304.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSQDVVDLD FFTQEPLHLV SPSFLSVTID ANLATDPRFL ILLGSPKLRT LARGLSPAYL RFGGTKTDFL IFDPKKESTF EERSYWQSQV NQDICKYGSI PPDVEEKLRL EWPYQEQLLL REHYQKKFKN STYSRSSVDV LYTFANCSGL DLIFGLNALL RTADLQWNSS NAQLLLDYCS SKGYNISWEL GNEPNSFLKK ADIFINGSQL GEDFIQLHKL LRKSTFKNAK LYGPDVGQPR RKTAKMLKSF LKAGGEVIDS VTWHHYYLNG RTATKEDFLN PDVLDIFISS VQKVFQVVES TRPGKKVWLG ETSSAYGGGA PLLSDTFAAG FMWLDKLGLS ARMGIEVVMR QVFFGAGNYH LVDENFDPLP DYWLSLLFKK LVGTKVLMAS VQGSKRRKLR VYLHCTNTDN PRYKEGDLTL YAINLHNVTK YLRLPYPFSN KQVDKYLLRP LGPHGLLSKS VQLNGLTLKM VDDQTLPPLM EKPLRPGSSL GLPAFSYSFF VIRNAKVAAC I
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: HPSE protein solution (1 mg/ml) containing 20mM Tris-HCl (pH 8.0), 10% glycerol and 0.4M Urea.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human HPSE General Information | |
|---|---|
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| Alternate Names | |
| Heparanase, HPA, HSE1, HPA1, HPR1, HPSE1, heparanase-1, EC 3.2. 1.166, Endo-glucoronidase, HEP, Heparanase-1, Hpa1. | |
| Molecular Weight | |
| 60 kDa | |
| Chromosomal Location | |
| q21.23 on chromosome 4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HPSE |
| Entrez Gene ID | 10855 |
| UniProt ID(s) | Q9Y251 |
| COSMIC ID Link(s) | HPSE |
| KEGG Gene ID(s) | hsa:10855 |
| General Description of Recombinant Human HPSE. | |
| Human HPSE produced in E. Coli is a single, non-glycosylated polypeptide chain containing 531 amino acids (36-543 AA) and having a molecular mass of 60kDa. HPSE is fused to a 23 amino acid His-tag at N-terminus. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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