PDF DatasheetHuman HS3ST1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HS3ST1 based upon sequence from Human
Host: QP12313 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HS3ST1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12313.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: HS3ST1 protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol and 2mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human HS3ST1 General Information | |
|---|---|
| Alternate Names | |
| Heparan sulfate glucosamine 3-O-sulfotransferase 1, Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1, 3-OST-1, Heparan sulfate 3-O-sulfotransferase 1, h3-OST-1, HS3ST1, 3OST, 3OST1, HS3S1. | |
| Molecular Weight | |
| 36.2 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | O14792 |
| General Description of Recombinant Human HS3ST1. | |
| Human HS3ST1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 310 amino acids (21-307 AA) and having a molecular mass of 36.2kDa. HS3ST1 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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