PDF DatasheetHuman HSD17B1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HSD17B1 based upon sequence from Human
Host: QP12320 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HSD17B1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12320.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMARTVV LITGCSSGIG LHLAVRLASD PSQSFKVYAT LRDLKTQGRL WEAARALACP PGSLETLQLD VRDSKSVAAA RERVTEGRVD VLVCNAGLGL LGPLEALGED AVASVLDVNV VGTVRMLQAF LPDMKRRGSG RVLVTGSVGG LMGLPFNDVY CASKFALEGL CESLAVLLLP FGVHLSLIEC GPVHTAFMEK VLGSPEEVLD RTDIHTFHRF YQYLAHSKQV FREAAQNPEE VAEVFLTALR APKPTLRYFT TERFLPLLRM RLDDPSGSNY VTAMHREVFG DVPAKAEAGA EAGGGAGPGA EDEAGRGAVG DPELGDPPAA PQ
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: HSD17B1 solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human HSD17B1 General Information | |
|---|---|
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| Alternate Names | |
| Estradiol 17-beta-dehydrogenase 1, EC 1.1. 1.62, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E2DH, Placental 17-beta-hydroxysteroid dehydrogenase, HSD17B1, E17KSR, EDH17B1, EDH17B2, EDHB17, HSD17, SDR28C1. | |
| Molecular Weight | |
| 37.5 kDa | |
| Chromosomal Location | |
| q21.2 on chromosome 17 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HSD17B1 |
| Entrez Gene ID | 3292 |
| UniProt ID(s) | P14061 |
| COSMIC ID Link(s) | HSD17B1 |
| KEGG Gene ID(s) | hsa:3292 |
| General Description of Recombinant Human HSD17B1. | |
| Human HSD17B1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 352 amino acids (1-328 AA) and having a molecular mass of 37.5kDa. HSD17B1 is fused to a 24 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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