PDF DatasheetCanine IFNG Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant IFNG based upon sequence from Canine
Host: QP12378 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of IFNG was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12378.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSQAMFFKE IENLKEYFNA SNPDVSDGGS LFVDILKKWR EESDKTIIQS QIVSFYLKLF DNFKDNQIIQ RSMDTIKEDM LGKFLNSSTS KREDFLKLIQ IPVNDLQVQR KAINELIKVM NDLSPRSNLR KRKRSQNLFR GRRASK
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: IFNG protein solution (0.5 mg/ml) contains 20mM MES (pH6.0), 20% glycerol, 0.1M NaCl and 1mM EDTA.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Canine IFNG General Information | |
|---|---|
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| Alternate Names | |
| Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma. | |
| Molecular Weight | |
| 19.3 kDa | |
| Chromosomal Location | |
| On chromosome 10 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IFNG |
| Entrez Gene ID | 403801 |
| UniProt ID(s) | P42161 |
| COSMIC ID Link(s) | IFNG |
| KEGG Gene ID(s) | cfa:403801 |
| General Description of Recombinant Canine IFNG. | |
| IFNG Canine Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 166 amino acids (24-166 AA) and having a molecular mass of 19.3kDa. IFNG is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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