PDF DatasheetCanine IL6 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant IL6 based upon sequence from Canine
Host: QP12427 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of IL6 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12427.
Bioactivity Data: Untested
Amino Acid Sequence: FPTPGPLAGD SKDDATSNSL PLTSANKVEE LIKYILGKIS ALRKEMCDKF NKCEDSKEAL AENNLHLPKL EGKDGCFQSG FNQETCLTRI TTGLVEFQLH LNILQNNYEG DKENVKSVHM STKILVQMLK SKVKNQDEVT TPDPTTDASL QAILQSQDEC VKHTTIHLIL RSLEDFLQFS LRAVRIMLEH HHHHH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: IL6 protein solution (0.5 mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Canine IL6 General Information | |
|---|---|
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| Alternate Names | |
| IL6, IL-6, Interleukin-6. | |
| Molecular Weight | |
| 22.0 kDa | |
| Chromosomal Location | |
| On chromosome 14 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IL6 |
| Entrez Gene ID | 403985 |
| UniProt ID(s) | P41323 |
| COSMIC ID Link(s) | IL6 |
| KEGG Gene ID(s) | cfa:403985 |
| General Description of Recombinant Canine IL6. | |
| IL6 Canine Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 195 amino acids (21-207AA ) and having a molecular mass of 22.0kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). IL6 is expressed with an 8 amino acid His-tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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