PDF DatasheetHuman IRF 1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant IRF 1 based upon sequence from Human
Host: QP12456 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of IRF 1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12456.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPITRMRMRP WLEMQINSNQ IPGLIWINKE EMIFQIPWKHAAKHGWDINK DACLFRSWAI HTGRYKAGEK EPDPKTWKAN FRCAMNSLPD IEEVKDQSRN KGSSAVRVYR MLPP
Purity: Greater than 90.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: 1 mg/ml in 20mM Tris pH 8 and 10% glycerol.
Storage Conditions: Liquid Interferon although stable at 10°C for 1 week, should be stored below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human IRF 1 General Information | |
|---|---|
| Alternate Names | |
| IRF-1, IRF1, MAR. | |
| Molecular Weight | |
| 15 kDa | |
| Chromosomal Location | |
| q31.1 on chromosome 5 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IRF1 |
| Entrez Gene ID | 3659 |
| UniProt ID(s) | P10914 |
| COSMIC ID Link(s) | IRF1 |
| KEGG Gene ID(s) | hsa:3659 |
| General Description of Recombinant Human IRF 1. | |
| Human Interferon Regulatory Factor-1 produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids (1-114) with a His Tag of 20 aa, and having a molecular mass of 15 kDa. The IRF-1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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