PDF DatasheetHuman ISG15 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ISG15 based upon sequence from Human
Host: QP12460 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of ISG15 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12460.
Bioactivity Data: Untested
Amino Acid Sequence: MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SMGWDLTVKM LAGNEFQVSL SSSMSVSELK AQITQKIGVH AFQQRLAVHP SGVALQDRVP LASQGLGPGS TVLLVVDKCD EPLNILVRNN KGRSSTYEVR LTQTVAHLKQ QVSGLEGVQD DLFWLTFEGK PLEDQLPLGE YGLKPLSTVF MNLRLRGG
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The ISG15 solution contains 20mM Tris-HCl pH 8.0, 100mM NaCl and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human ISG15 General Information | |
|---|---|
| Alternate Names | |
| ISG15, G1P2, IFI15, UCRP, ISG15 Ubiquitin-like modifier. | |
| Molecular Weight | |
| 34 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P05161 |
| General Description of Recombinant Human ISG15. | |
| Human ISG15 fused to N-terminal Calmodulin Tag (151 AA ) produced in E. Coli is a single, non-glycosylated polypeptide chain containing a total of 308 amino acids (157 AA without tag) and having a molecular mass of 34 kDa. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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