PDF DatasheetHuman ITGB1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ITGB1 based upon sequence from Human
Host: QP12462 protein expressed in E. Coli, Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of ITGB1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12462.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQTDENRCLK ANAKSCGECI QAGPNCGWCT NSTFLQEGMP TSARCDDLEA LKKKGCPPDD IENPRGSKDI KKNKNVTNRS KGTAEKLKPE DITQIQPQQL VLRLRSGEPQ TFTLKFKRAE DYPIDLYYLM DLSYSMKDDL ENVKSLGTDL MNEMRRITSDFRIGFGSFVE KTVMPYISTT PAKLRNPCTS EQNCTSPFSY KNVLSLTNKG EVFNELVGKQ RISGNLDSPE GGFDAIMQVA VCGSLIGWRN VTRLLVFSTD AGFHFAGDGK LGGIVLPNDG QCHLENNMYT MSHYYDYPSI AHLVQKLSEN NIQTIFAVTE EFQPVYKELK NLIPKSAVGT LSANSSNVIQ LIIDAYNSLS SEVILENGKL SEGVTISYKS YCKNGVNGTG ENGRKCSNIS IGDEVQFEIS ITSNKCPKKD SDSFKIRPLG FTEEVEVILQ YI
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The ITGB1 solution (1 mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human ITGB1 General Information | |
|---|---|
| Alternate Names | |
| Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29, ITGB1, FNRB, MDF2, MSK12, Integrin beta 1, CD29, VLAB. | |
| Molecular Weight | |
| 51.2 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P05556 |
| General Description of Recombinant Human ITGB1. | |
| Human ITGB1 produced in E. coli is a single polypeptide chain containing 462 amino acids (21-461) and having a molecular mass of 51.2 kDa. ITGB1 is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
Induction of Endothelial CellProliferation by Recombinant and Microparticle-Tissue Factor Involves b1-Integrinand Extracellular Signal Regulated Kinase ActivationPublication: Arteriosclerosis, thrombosis, and vascularbiology 30.9 (2010): 1810-1817.Link: http://atvb.ahajournals.org/content/30/9/1810.full
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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