PDF DatasheetHuman LIPG Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant LIPG based upon sequence from Human
Host: QP12577 protein expressed in E. Coli, HEK 293.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of LIPG was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12577.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSPVPFGP EGRLEDKLHK PKATQTEVKP SVRFNLRTSK DPEHEGCYLS VGHSQPLEDC SFNMTAKTFF IIHGWTMSGI FENWLHKLVS ALHTREKDAN VVVVDWLPLA HQLYTDAVNN TRVVGHSIAR MLDWLQEKDD FSLGNVHLIG YSLGAHVAGY AGNFVKGTVG RITGLDPAGP MFEGADIHKR LSPDDADFVD VLHTYTRSFG LSIGIQMPVG HIDIYPNGGD FQPGCGLNDV LGSIAYGTIT EVVKCEHERA VHLFVDSLVN QDKPSFAFQC TDSNRFKKGI CLSCRKNRCN SIGYNAKKMR NKRNSKMYLK TRA
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The LIPG solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human LIPG General Information | |
|---|---|
| Alternate Names | |
| LIPG, Lipase Endothelial, EDL, EL, Endothelial Cell-Derived Lipase, EC 3.1. 1.3, PRO719, Endothelial Lipase, Lipoprotein Lipase H, EC 3.1. 1. | |
| Molecular Weight | |
| 38 kDa | |
| Chromosomal Location | |
| q21.1 on chromosome 18 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LIPG |
| Entrez Gene ID | 9388 |
| UniProt ID(s) | Q9Y5X9 |
| COSMIC ID Link(s) | LIPG |
| KEGG Gene ID(s) | hsa:9388 |
| General Description of Recombinant Human LIPG. | |
| Human LIPG produced in E. coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (21-340) and having a molecular mass of 38kDa. LIPG is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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