PDF DatasheetHuman MAPK12 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MAPK12 based upon sequence from Human
Host: QP12641 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of MAPK12 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12641.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSPPPARSG FYRQEVTKTA WEVRAVYRDL QPVGSGAYGA VCSAVDGRTG AKVAIKKLYR PFQSELFAKR AYRELRLLKH MRHENVIGLL DVFTPDETLD DFTDFYLVMP FMGTDLGKLM KHEKLGEDRI QFLVYQMLKG LRYIHAAGII HRDLKPGNLA VNEDCELKIL DFGLARQADS EMTGYVVTRW YRAPEVILNW MRYTQTVDIW SVGCIMAEMI TGKTLFKGSD HLDQLKEIMK VTGTPPAEFV QRLQSDEAKN YMKGLPELEK KDFASILTNA SPLAVNLLEK MLVLDAEQRV TAGEALAHPY FESLHDTEDE PQVQKYDDSF DDVDRTLDEW KRVTYKEVLS FKPPRQLGAR VSKETPL
Purity: Greater than 95.0% as determined by SDS-PAGE analysis.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: MAPK12 protein solution (0.5 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human MAPK12 General Information | |
|---|---|
| Alternate Names | |
| Mitogen-activated protein kinase 12, SAPK3, ERK6, PRKM12, Extracellular signal-regulated kinase 6, Mitogen-activated protein kinase p38 gamma, Stress-activated protein kinase 3, MAP kinase 12, MAP kinase p38 gamma, MAPK 12, ERK3, p38gamma, EC 2.7. 11.24, EC 2.7. 11. | |
| Molecular Weight | |
| 44.1 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P53778 |
| General Description of Recombinant Human MAPK12. | |
| Human MAPK12 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 387 amino acids (1-367 AA ) and having a molecular mass of 44.1kDa. MAPK12 is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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