PDF DatasheetHuman MCEE Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MCEE based upon sequence from Human
Host: QP12659 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of MCEE was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12659.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQVTGSVWNL GRLNHVAIAV PDLEKAAAFY KNILGAQVSE AVPLPEHGVS VVFVNLGNTK MELLHPLGRD SPIAGFLQKN KAGGMHHICI EVDNINAAVM DLKKKKIRSL SEEVKIGAHG KPVIFLHPKD CGGVLVELEQ A
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The MCEE protein solution (1 mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT, 0.1mM PMSF and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human MCEE General Information | |
|---|---|
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| Alternate Names | |
| GLOD2, Methylmalonyl CoA Epimerase, Glyoxalase Domain Containing 2, DL-methylmalonyl-CoA Racemase. | |
| Molecular Weight | |
| 17.3 kDa | |
| Chromosomal Location | |
| p13.3 on chromosome 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MCEE |
| Entrez Gene ID | 84693 |
| UniProt ID(s) | Q96PE7 |
| COSMIC ID Link(s) | MCEE |
| KEGG Gene ID(s) | hsa:84693 |
| General Description of Recombinant Human MCEE. | |
| MCEE produced in E. Coli is a single, non-glycosylated polypeptide chain containing 161 amino acids (37-176AA ) and having a molecular mass of 17.3kDa. MCEE is fused to a 21 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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