PDF DatasheetE. Coli MSRB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MSRB based upon sequence from E. Coli
Host: QP12743 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of MSRB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12743.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MANKPSAEEL KKNLSEMQFY VTQNHGTEPP FTGRLLHNKR DGVYHCLICD APLFHSQTKY DSGCGWPSFY EPVSEESIRY IKDLSHGMQR IEIRCGNCDA HLGHVFPDGP QPTGERYCVN SASLRFTDGE NGEEING
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: MSRB protein solution (1 mg/ml) 20mM Tris-HCl buffer (pH 8.0), 20% glycerol 0.1M NaCl and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant E. Coli MSRB General Information | |
|---|---|
| Alternate Names | |
| Peptide methionine sulfoxide reductase MsrB, Peptide-methionine (R)-S-oxide reductase, msrB, yeaA, b1778, JW1767. | |
| Molecular Weight | |
| 17.6 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P0A746 |
| General Description of Recombinant E. Coli MSRB. | |
| MSRB produced in E. Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids (1-137 AA ) and having a molecular mass of 17.6kDa. MSRB is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.