PDF DatasheetHuman MYL12B Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MYL12B based upon sequence from Human
Host: QP12768 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of MYL12B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12768.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSSKKA KTKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DAYLDAMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEATG TIQEDYLREL LTTMGDRFTD EEVDELYREA PIDKKGNFNY IEFTRILKHG AKDKDD
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The MYL12B solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM Nacl, 2mM DTT and 40% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human MYL12B General Information | |
|---|---|
| Alternate Names | |
| Myosin light chain 12B regulatory, Myosin regulatory light chain 2-B smooth muscle isoform, Myosin regulatory light chain 20 kDa, Myosin regulatory light chain MRLC2, MYLC2B, MRLC2, MLC-2A, MLC-B, SHUJUN-1 MLC20. | |
| Molecular Weight | |
| 22.3 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | O14950 |
| General Description of Recombinant Human MYL12B. | |
| Human MYL12B produced in E. coli is a single polypeptide chain containing 196 amino acids (1-172) and having a molecular mass of 22.3kDa. MYL12B is fused to a 24 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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