PDF DatasheetHuman NCK1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant NCK1 based upon sequence from Human
Host: QP12801 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of NCK1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12801.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVIEKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP IFTSEQGEKL YLVKHLS
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: NCK1 Human solution containing 20mM Tris-HCl pH 8.0, 1mM DTT, 1mM EDTA, 50mM NaCl & 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human NCK1 General Information | |
|---|---|
| Alternate Names | |
| NCK, NCKalpha, NCK1, NCK adaptor protein 1, Cytoplasmic protein NCK1, SH2/SH3 adaptor protein NCK-alpha, MGC12668. | |
| Molecular Weight | |
| 45 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P16333 |
| General Description of Recombinant Human NCK1. | |
| Human NCK1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 397 amino acids (1-377 AA ) and having a molecular mass of 45 kDa. The NCK1 is fused to a 20 amino acid His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used for any other purpose. This product is guaranteed to work for a period of one year when stored at -70°C or colder.
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