PDF DatasheetHuman NDUFA5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant NDUFA5 based upon sequence from Human
Host: QP12812 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of NDUFA5 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12812.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGVLKK TTGLVGLAVC NTPHERLRIL YTKILDVLEE IPKNAAYRKY TEQITNEKLA MVKAEPDVKK LEDQLQGGQL EEVILQAEHE LNLARKMREW KLWEPLVEEP PADQWKWPI
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The NDUFA5 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human NDUFA5 General Information | |
|---|---|
| Alternate Names | |
| NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, Complex I subunit B13, Complex I-13kD-B, CI-13kD-B, NADH-ubiquinone oxidoreductase 13 kDa-B subunit, NDUFA5, B13, NUFM, UQOR13, CI-13kB. | |
| Molecular Weight | |
| 15.8 kDa | |
| Chromosomal Location | |
| q31.32 on chromosome 7 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NDUFA5 |
| Entrez Gene ID | 4698 |
| UniProt ID(s) | Q16718 |
| COSMIC ID Link(s) | NDUFA5 |
| KEGG Gene ID(s) | hsa:4698 |
| General Description of Recombinant Human NDUFA5. | |
| Human NDUFA5 produced in E. coli is a single, non-glycosylated polypeptide chain containing 139 amino acids (1-116) and having a molecular mass of 15.8kDa. NDUFA5 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.