PDF DatasheetHuman NECAP2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant NECAP2 based upon sequence from Human
Host: QP12823 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of NECAP2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12823.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST SSQTQPGTGW VQF
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The NECAP2 protein solution (1 mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 2mM DTT and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human NECAP2 General Information | |
|---|---|
| Alternate Names | |
| NECAP endocytosis-associated protein 2, NECAP-2, FLJ10420, Adaptin ear-binding coat-associated protein 2. | |
| Molecular Weight | |
| 30.5 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9NVZ3 |
| General Description of Recombinant Human NECAP2. | |
| NECAP2 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 283 amino acids (1-263AA ) and having a molecular mass of 30.5 kDa. NECAP2 is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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