PDF DatasheetHuman NT5C3B Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant NT5C3B based upon sequence from Human
Host: QP12889 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of NT5C3B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12889.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEEVST LMKATVLMRQ PGRVQEIVGA LRKGGGDRLQ VISDFDMTLS RFAYNGKRCP SSYNILDNSK IISEECRKEL TALLHHYYPI EIDPHRTVKE KLPHMVEWWT KAHNLLCQQK IQKFQIAQVV RESNAMLREG YKTFFNTLYH NNIPLFIFSA GIGDILEEII RQMKVFHPNI HIVSNYMDFN EDGFLQGFKG QLIHTYNKNS SACENSGYFQ QLEGKTNVIL LGDSIGDLTM ADGVPGVQNI LKIGFLNDKV EERRERYMDS YDIVLEKDET LDVVNGLLQH ILCQGVQLEM QGP
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: NT5C3B protein solution (1 mg/ml) containing Phosphate Buffered Saline (pH 7.4), 20% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human NT5C3B General Information | |
|---|---|
| Alternate Names | |
| NT5C3L, 7-methylguanosine phosphate-specific 5′-nucleotidase, Cytosolic 5′-nucleotidase 3B, Cytosolic 5′-nucleotidase III-like protein, cN-III-like protein, N(7)-methylguanylate 5′-phosphatase. | |
| Molecular Weight | |
| 36.8 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q969T7 |
| General Description of Recombinant Human NT5C3B. | |
| Human NT5C3B produced in E. Coli is a single, non-glycosylated polypeptide chain containing 323 amino acids (1-300 AA) and having a molecular mass of 36.8kDa. NT5C3B is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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