PDF DatasheetHuman NT5M Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant NT5M based upon sequence from Human
Host: QP12890 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of NT5M was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12890.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: NT5M protein solution (0.5 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human NT5M General Information | |
|---|---|
| Alternate Names | |
| 5′(3′)-deoxyribonucleotidase mitochondrial, 5′,3′-nucleotidase, mitochondrial, Deoxy-5′-nucleotidase 2, dNT-2, NT5M, DNT2. | |
| Molecular Weight | |
| 25.1 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9NPB1 |
| General Description of Recombinant Human NT5M. | |
| Human NT5M produced in E. Coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (32-228 AA ) and having a molecular mass of 25.1kDa. NT5M is fused to a 21 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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