PDF DatasheetHuman PCNP Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PCNP based upon sequence from Human
Host: QP12978 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PCNP was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12978.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMADGKA GDEKPEKSQR AGAAGGPEEE AEKPVKTKTV SSSNGGESSS RSAEKRSAEE EAADLPTKPT KISKFGFAIG SQTTKKASAI SIKLGSSKPK ETVPTLAPKT LSVAAAFNED EDSEPEEMPP EAKMRMKNIG RDTPTSAGPN SFNKGKHGFS DNQKLWERNI KSHLGNVHDQ DN
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PCNP protein solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human PCNP General Information | |
|---|---|
| Alternate Names | |
| PEST proteolytic signal-containing nuclear protein, PCNP, PEST-containing nuclear protein. | |
| Molecular Weight | |
| 21.5 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q8WW12 |
| General Description of Recombinant Human PCNP. | |
| Human PCNP produced in E. Coli is a single, non-glycosylated polypeptide chain containing 202 amino acids (1-178 AA ) and having a molecular mass of 21.5kDa. PCNP is fused to a 24 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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