PDF DatasheetHuman PDE6D Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PDE6D based upon sequence from Human
Host: QP12990 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PDE6D was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12990.
Bioactivity Data: Untested
Amino Acid Sequence: MSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV GSHHHHHH
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The PDE6D protein solution contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1mM DTT and 100mM NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human PDE6D General Information | |
|---|---|
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| Alternate Names | |
| Retinal rod rhodopsin-sensitive cGMP 3′,5′-cyclic phosphodiesterase subunit delta, Phosphodiesterase 6D cGMP-Specific Rod Delta, GMP-PDE delta, Protein p17, PDE6D, PDED. | |
| Molecular Weight | |
| 18.4 kDa | |
| Chromosomal Location | |
| q37.1 on chromosome 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PDE6D |
| Entrez Gene ID | 5147 |
| UniProt ID(s) | O43924 |
| COSMIC ID Link(s) | PDE6D |
| KEGG Gene ID(s) | hsa:5147 |
| General Description of Recombinant Human PDE6D. | |
| PDE6D produced in E. Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (1-150 AA ) and having a molecular mass of 18.4kDa. PDE6D is fused to an 8 amino acids His Tag at C-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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