PDF DatasheetHuman PDE6H Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PDE6H based upon sequence from Human
Host: QP12991 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PDE6H was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12991.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSDNTTL PAPASNQGPT TPRKGPPKFK QRQTRQFKSK PPKKGVKGFG DDIPGMEGLG TDITVICPWE AFSHLELHEL AQFGII
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PDE6H protein solution (0.5 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, 2mM DTT and 0.1mM PMSF.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human PDE6H General Information | |
|---|---|
| Alternate Names | |
| Phosphodiesterase 6H, CGMP-Specific, Cone, Gamma, Retinal Cone Rhodopsin-Sensitive CGMP 3′,5′-Cyclic Phosphodiesterase, Subunit Gamma, EC 3.1. 4.35, EC 3.1. 4.17, RCD3, GMP-PDE Gamma, ACHM6, PDE6H. | |
| Molecular Weight | |
| 11.5 kDa | |
| Chromosomal Location | |
| p12.3 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PDE6H |
| Entrez Gene ID | 5149 |
| UniProt ID(s) | Q13956 |
| COSMIC ID Link(s) | PDE6H |
| KEGG Gene ID(s) | hsa:5149 |
| General Description of Recombinant Human PDE6H. | |
| Human PDE6H produced in E. Coli is a single, non-glycosylated polypeptide chain containing 106 amino acids (1-83 AA) and having a molecular mass of 11.5 kDa. PDE6H is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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