PDF DatasheetHuman PDGFRA Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PDGFRA based upon sequence from Human
Host: QP12993 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PDGFRA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12993.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQLSLPSILP NENEKVVQLN SSFSLRCFGE SEVSWQYPMS EEESSDVEIR NEENNSGLFV TVLEVSSASA AHTGLYTCYY NHTQTEENEL EGRHIYIYVP DPDVAFVPLG MTDYLVIVED DDSAIIPCRT TDPETPVTLH NSEGVVPASY DSRQGFNGTF TVGPYICEAT VKGKKFQTIP FNVYALKATS ELDLEMEALK TVYKSGETIV VTCAVFNNEV VDLQWTYPGE VKGKGITMLE EIKVPSIKLV YTLTVPEATV KDSGDYECAA RQATREVKEM KKVTISVHEK GFIEIKPTFS QLEAVNLHEV KHFVVEVRAY PPPRISWLKN NLTLIENLTE ITTDVEKIQE IRYRSKLKLI RAKEEDSGHY TIVAQNEDAV KSYTFELLTQ VPSSILDLVD DHHGSTGGQT VRCTAEGTPL PDIEWMICKD IKKCNNETSW TILANNVSNI ITEIHSRDRS TVEGRVTFAK VEETIAVRCL AKNLLGAENR ELKLVAPTLR SE
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The PDGFRA solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human PDGFRA General Information | |
|---|---|
| Alternate Names | |
| Platelet-derived growth factor receptor alpha polypeptide, PDGFR2, PDGF-R-alpha, CD140 antigen-like family member A, CD140a antigen, alpha-type platelet-derived growth factor receptor, RHEPDGFRA, rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha, PDGFRA/BCR fusion protein, MGC74795, EC 2.7. 10.1. | |
| Molecular Weight | |
| 58.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P16234 |
| General Description of Recombinant Human PDGFRA. | |
| Human PDGFRA produced in E. coli is a single, non-glycosylated polypeptide chain containing 522 amino acids (24-524) and having a molecular mass of 58.4 kDa. The PDGFRA is fused to a 20 amino acid His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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