PDF DatasheetHuman PDHX Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PDHX based upon sequence from Human
Host: QP12994 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PDHX was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12994.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSGDPIKIL MPSLSPTMEE GNIVKWLKKE GEAVSAGDAL CEIETDKAVV TLDASDDGIL AKIVVEEGSK NIRLGSLIGL IVEEGEDWKH VEIPKDVGPP PPVSKPSEPR PSPEPQISIP VKKEHIPGTL RFRLSPAARN ILEKHSLDAS QGTATGPRGI FTKEDALKLV QLKQTGKITE SRPTPAPTAT PTAPSPLQAT AGPSYPRPVI PPVSTPGQPN AVGTFTEIPA SNIRRVIAKR LTESKSTVPH AYATADCDLG AVLKVRQDLV KDDIKVSVND FIIKAAAVTL KQMPDVNVSW DGEGPKQLPF IDISVAVATD KGLLTPIIKD AAAKGIQEIA DSVKALSKKA RDGKLLPEEY QGGSFSISNL GMFGIDEFTA VINPPQACIL AVGRFRPVLK LTEDEEGNAK LQQRQLITVT MSSDSRVVDD ELATRFLKSF KANLENPIRL A
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PDHX protein solution (0.25 mg/ml) containing Phosphate buffer saline (pH 7.4) and 20% glycerol, 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human PDHX General Information | |
|---|---|
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| Alternate Names | |
| DLDBP, E3BP, OPDX, PDX1, proX, Pyruvate Dehydrogenase Complex, Component X, Dihydrolipoamide Dehydrogenase-Binding Protein Of Pyruvate Dehydrogenase Complex, Lipoyl-Containing Pyruvate Dehydrogenase Complex Component X, Pyruvate Dehydrogenase Complex, Lipoyl-Containing Component X, Pyruvate Dehydrogenase Complex, E3-Binding Protein Subunit, Pyruvate Dehydrogenase Protein X Component, Mitochondrial, E3-Binding Protein. | |
| Molecular Weight | |
| 50.4 kDa | |
| Chromosomal Location | |
| p13 on chromosome 11 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PDHX |
| Entrez Gene ID | 8050 |
| UniProt ID(s) | O00330 |
| COSMIC ID Link(s) | PDHX |
| KEGG Gene ID(s) | hsa:8050 |
| General Description of Recombinant Human PDHX. | |
| Human PDHX produced in E. Coli is a single, non-glycosylated polypeptide chain containing 471 amino acids (54-501 AA) and having a molecular mass of 50.4kDa. PDHX is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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