PDF DatasheetHuman PDIA3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PDIA3 based upon sequence from Human
Host: QP12995 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PDIA3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12995.
Bioactivity Data: Untested
Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The PDIA3 protein solution contains 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M NaCl and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human PDIA3 General Information | |
|---|---|
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| Alternate Names | |
| ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC, ER60, Protein disulfide-isomerase A3, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 60, ER protein 60, 58 kDa microsomal protein, Endoplasmic reticulum resident protein 57, ER protein 57, 58 kDa glucose-regulated protein, PDIA3. | |
| Molecular Weight | |
| 58.5 kDa | |
| Chromosomal Location | |
| q15.3 on chromosome 15 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PDIA3 |
| Entrez Gene ID | 2923 |
| UniProt ID(s) | P30101 |
| COSMIC ID Link(s) | PDIA3 |
| KEGG Gene ID(s) | hsa:2923 |
| General Description of Recombinant Human PDIA3. | |
| Human PDIA3 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 518 amino acids (25-505 AA ) and having a molecular weight of 58.5 kDa. The PDIA3 is fused to 37 AA His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
The dehydrogenase region of the NADPH oxidase component Nox2 acts as a protein disulfide isomerase (PDI) resembling PDIA3 with a role in the binding of the activator protein p67phoxPublications: Frontiers in Chemistry 3.3 (2015).Link:http://journal.frontiersin.org/article/10.3389/fchem.2015.00003/full
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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