PDF DatasheetHuman PINK1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PINK1 based upon sequence from Human
Host: QP13056 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of PINK1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13056.
Bioactivity Data: Untested
Amino Acid Sequence: MYLIGQSIGK GCSAAVYEAT MPTLPQNLEV TKSTGLLPGR GPGTSAPGEG QERAPGAPAF PLAIKMMWNI SAGSSSEAIL NTMSQELVPA SRVALAGEYG AVTYRKSKRG PKQLAPHPNI IRVLRAFTSS VPLLPGALVD YPDVLPSRLH PEGLGHGRTL FLVMKNYPCT LRQYLCVNTP SPRLAAMMLL QLLEGVDHLV QQGIAHRDLK SDNILVELDP DGCPWLVIAD FGCCLADESI GLQLPFSSWY VDRGGNGCLM APEVSTARPG PRAVIDYSKA DAWAVGAIAY EIFGLVNPFY GQGKAHLESR SYQEAQLPAL PESVPPDVRQ LVRALLQREA SKRPSARVAA NVL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The PINK1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1M Urea and 5% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human PINK1 General Information | |
|---|---|
| Alternate Names | |
| PTEN induced putative kinase 1, PARK6, protein kinase BRPK, Parkinson disease (autosomal recessive) 6, serine/threonine-protein kinase PINK1 mitochondrial, EC 2.7. 11.1. | |
| Molecular Weight | |
| 37.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9BXM7 |
| General Description of Recombinant Human PINK1. | |
| Human PINK1 produced in E. coli is a single, non-glycosylated polypeptide chain containing 353 amino acids (156-507) and having a molecular mass of 37.9 kDa. PINK1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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