PDF DatasheetHuman PKIB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PKIB based upon sequence from Human
Host: QP13065 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PKIB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13065.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRTDSSKMTD VESGVANFAS SARAGRRNAL PDIQSSAATD GTSDLPLKLE ALSVKEDAKE KDEKTTQDQL EKPQNEEK
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PKIB solution (1 mg/ml) 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human PKIB General Information | |
|---|---|
| Alternate Names | |
| cAMP-dependent protein kinase inhibitor beta, PKI-beta, PKIB, PRKACN2, FLJ23817. | |
| Molecular Weight | |
| 10.6 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9C010 |
| General Description of Recombinant Human PKIB. | |
| Human PKIB produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 98 amino acids (1-78) and having a molecular mass of 10.6kDa (Molecular size on SDS-PAGE will appear higher). PKIB is fused to a 20 aa His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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